Label-free 3D imaging of microstructure, blood and lymphatic vessels within tissue beds in vivo
Identifieur interne : 004204 ( Main/Exploration ); précédent : 004203; suivant : 004205Label-free 3D imaging of microstructure, blood and lymphatic vessels within tissue beds in vivo
Auteurs : Zhongwei Zhi ; Yeongri Jung ; Ruikang K. WangSource :
- Optics letters [ 0146-9592 ] ; 2012.
Abstract
This letter reports the use of an ultrahigh resolution optical microangiography (OMAG) system for simultaneous 3D imaging of microstructure, lymphatic and blood vessels without the use of exogenous contrast agent. An automatic algorithm is developed to segment the lymphatic vessels from the microstructural images, based on the fact that the lymph fluid is optically transparent. The OMAG system is developed that utilizes a broadband supercontinuum light source, providing an axial resolution of 2.3 μm and lateral resolution of 5.8 μm, capable of resolving the capillary vasculature and lymphatic vessels innervating microcirculatory tissue beds. Experimental demonstration is performed by showing detailed 3D lymphatic and blood vessel maps, coupled with morphology, within mouse ears
Url:
PubMed: 22378402
PubMed Central: 3980723
Affiliations:
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Le document en format XML
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